脑多头蚴重组蛋白rTm16和rTm-GST的免疫保护效果分析

doi:10.11843/j.issn.0366-6964.2018.12.020

Abstract

Abstract:

To explore the immune protective effect of recombinant Tm16 and GST proteins against Coenurus cerebralis in goats, forty-four goats were divided into four groups, including rTm16 group, rTm-GST group, rTm16+rTm-GST group and control group, which were immunized 3 times by subcutaneous injection, respectively. Second vaccination was performed 4 weeks after the first vaccination, and 6 months later the last vaccination was performed. The serum was regularly collected from each goat, and the specific IgG response to each group was measured by indirect ELISA. Two weeks after the final vaccination, each goat was orally challenged with 5 500 eggs of Taenia multiceps. One hundred and five days post infection all goats were slaughtered, the cysts in brain and muscle were collected and counted. Result were as follows:Compared with the control group, the cysts of goat in group rTm16, rTm-GST and rTm16+rTm-GST reduced by 50%, 62.5% and 87.5%, respectively. Combined immunization of rTm16 with rTm-GST induced significant protection (P<0.05)against challenge of T. multiceps in goats. Result of iELISA showed that the specific IgG response against rTm-GST was significantly weaker than that against rTm16 (P < 0.05). However, the specific IgG response against rTm-GST was not significant compared with that against rTm16 in combined group (P>0.05). This revealed that the combined use of rTm16 and rTm-GST could induce higher antibody levels in goats, thus obtaining significant protective immunity.

CLC Number:

S852.734

GUO Cheng, WAN Jie, YANG Ying-dong, WEN Jian-guo, LIU Yu-chen, GU Xiao-bin, XIE Yue, YANG Guang-you. Immune Protective Effect Analysis of Recombinant Protein rTm16 and rTm-GST from Coenurus cerebralis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2707-2714.

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Immune Protective Effect Analysis of Recombinant Protein rTm16 and rTm-GST from Coenurus cerebralis

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